Evolution of mitochondria
Mitochondria are the power stations of human cells. They provide the energy needed for the cellular metabolism. But how did these power stations evolve, and how are they constructed? Researchers from the University of Freiburg studied the role of so-called oxidase assembly machinery, or OXA, in the development of the inner membrane of mitochondria and the energy supply of cells. Dr. Jan Hopker, Dr. Silke Oeljeklaus, Prof. Dr. Nikolaus Pfanner, Dr. Sebastian Stiller, Prof. Dr. Bettina Warscheid, Prof. Dr. Nils Wiedemann and their team of researchers have demonstrated that this protein complex is essential for the integration of certain proteins into the inner membrane of mitochondria - proteins that play a role in cellular respiration and other processes. The results of the scientists' research have now been published in the journal Cell Metabolism.
|Fluorescence microscopy image of the mitochondrial network (left, in green) and the corresponding light microscopy |
image (right) of a dividing yeast cell [Credit: Nils Wiedemann/University of Freiburg]
The researchers from the University of Freiburg systematically searched for proteins integrated by OXA into the inner membrane after they had been imported via TOM and TIM. They used an analytical technique called quantitative mass spectrometry to identify mitochondrial inner membrane proteins which are reduced in cells without OXA. By tracing the integration of radioactively labelled proteins into the inner mitochondrial membrane, they were able to prove that OXA is necessary for this process.
The imported OXA-dependent proteins play important functions that range from cellular respiration, the exchange of metal ions, and biochemical reactions, to the integration of proteins enabling the transfer of metabolic products across the inner membrane. When the integration or function of these respiratory proteins is blocked, this can cause mitochondrial-based neuromuscular diseases or cancer. The OXA-dependent integration of inner membrane proteins, which has been conserved throughout evolution, is thus fundamental for the formation of the mitochondrial inner membrane and for the energy supply of human cells.
Source: Albert Ludwigs University of Freiburg [May 18, 2016]